Soul

The soul, in many mythological, religious, philosophical, and psychological traditions, is the incorporeal and, in many conceptions, immortal essence of a person, living thing, or object.

In MediEvil, souls of Dan's vanquished enemies fill the Chalice. These souls are marked as bad on the Wiki.

Some enemies Daniel encounters are soulless, however, and killing them does not fill the Chalice.
There are also enemies with good souls. Killing such enemies drains the Chalice of its power instead.

Enemies with good souls

MediEvil and MediEvil: Resurrection

File:MadEnem.png	Mr. Mad Mr. Mad was once a shy, gentle and educated man, but boy, how things have changed since his enchantment! This gibbering mess now staggers around rudely brandishing a large sharp axe, and yes, it seems he will use it at any given opportunity.
File:MrsMadEnem.png	Mrs. Mad This frumpy gal is a surprisingly fearsome opponent. Enchantment seems to have multiplied her strength tenfold and being smacked forcefully in the face with a frying pan is never a pleasant experience.
File:NellieEnem.png	Nellie Mad Never before in the history of Gallowmere has such a sweet little girlie presented such a terrifying menace to society - such is the dark power of Zarok's enchantment! Suffice it to say, her speedy jump attacks with hatchet are best dodged at all times.